Typing of Human Papillomavirus by Pyrosequencing

Human Papillomavirus Typing in HIV-Positive Women

In this paper (page 89 of this issue), Hameed and colleagues set out to study the presence of lowand high-risk human papillomavirus (HPV) types in women infected by human immunodeficiency virus (HIV), and to correlate the presence of HPV with Pap smear results. It is not surprising to find HPV to be present in the cervices of HIV-infected women even though their Pap smears were not abnormal. Wh...

Human Papillomavirus Typing in HIV-Positive Women

OBJECTIVE Human papillomavirus (HPV) is the major cause of cervical carcinoma and cervical intraepithelial neoplasia worldwide. Certain HPV types have a strong association with and probably a causative role in the pathogenesis of premalignant cervical lesions. Epidemiologic studies in women infected by the human immunodeficiency virus (HIV) have shown an increased incidence of squamous intraepi...

Detection and typing of human pathogenic hantaviruses by real-time reverse transcription-PCR and pyrosequencing.

BACKGROUND Because the clinical course of human infections with hantaviruses can vary from subclinical to fatal, rapid and reliable detection of hantaviruses is essential. To date, the diagnosis of hantavirus infection is based mainly on serologic assays, and the detection of hantaviral RNA by the commonly used reverse transcription (RT)-PCR is difficult because of high sequence diversity of ha...

Lyssavirus Detection and Typing Using Pyrosequencing 1

Lyssavirus detection and typing using pyrosequencing.

Rabies is a fatal zoonosis caused by a nonsegmented negative-strand RNA virus, namely, rabies virus (RABV). Apart from RABV, at least 10 additional species are known as rabies-related lyssaviruses (RRVs), and some of them are responsible for occasional spillovers into humans. More lyssaviruses have also been detected recently in different bat ecosystems, thanks to the application of molecular d...